Temporal Report Outline

(based on WHO 6e, 2021)
Normal values are written in green highlight.

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Patient information:

Name, age, ID, address:

Abstinence duration (2-7d)

Microscopic examination

Clumping (Nil in normal)

Sperm aggregates [Non specific, mucin strands, other cells, debris] (none/some/plenty)

Agglutinates (Degrees: 1-isolated <10s/agg; 2-moderate 10-50s/agg; 3-large agg >50s/agg some free; 4-gross all s in interconnected agg)

Sperm count

Count/concentration (15-18 million/ml)

Motility

Total motility (>40%)

Progressive Rapid [25microm/s or more] (>29%)
25microm = 1/2 tail length

Slow Progressive [5-24microm/s] (10%)

Non Progressive [<5microm/s] (1%)

Immotile [no tail movement] (<20%)

Vitality [if motility <40%, assessed <60mins]

Total live (>50%)

Live motile: 

Live immotile ( <25% of all/ >25-30%: ciliary problem ~Kartagener)

Dead

Morphology

Normal (>3.9%)

Abnormal

• Head abnormality
• Neck and midpiece abnormality
• Tail abnormality
• Excess residual cytoplasm

Other Cells

WBC (<1 million/ml)

RBC (+/-)

Extended examination

Anti-sperm antibodies (if agglutination degree of 1 or more)

Semen Fructose (>13mic.moles/ejaculate)
Vas deferens and ejaculatory duct normal

Remarks: 

• Normozoospermia (all parameters normal)
• Oligozoospermia (count reduced)
• Azoospermia (absence of sperm in ejaculate)
• Aspermia (absence of ejaculate)
• Asthenozoospermia (Progressive rapid motility < 29%)
• Teratozoospermia (normal morphology <3.9%)
• Oligo-astheno-teratozoospermia (all parameters abnormal)
• Leukocytospermia (>1million/mL WBC)
• Necrozoospermia (All sperms non viable)

REPORTING FORMAT

Proposed Template

Test Parameter	Observed	Biological reference interval (95% CI)	Abnormal
Abstinence duration (days)		2-7 days	<2 days
Physical examination
Volume (ml)		1.3 – 1.5 mL	<1.3 mL
Liquefaction time (mins)		15 – 30 mins	>30mins, >60 mins enzymatic
Appearance		Grey opalescent	Grey opalescent / Slightly yellowish / Clear yellow / Reddish-brown / Clear-viscous
Viscosity		slight	slight / high
Odour		no abnormal smell	urine/putrid smell
pH		>7.2	<7.2
Microscopic examination
CLUMPING
Sperm aggregates		none	none/some/plenty
Agglutinates		nil	Degree 1/2/3/4
SPERM COUNT
Count/concentration (million/mL)		15-18 million/mL	<15 million/mL
MOTILITY
Total motility (RP+SP+NP)		>40%	<40%
Immotile		–	–
VITALITY
Total live		>50%	<50%
Dead		–	–
MORPHOLOGY
Normal		>3.9%	<3.9%
Abnormal (H+N+M+T)		–	–
OTHER CELLS
WBC (million/mL)		<1 million/mL	>1 million/mL
RBC		nil	+
Extended examination
Anti-sperm antibodies		negative	+
Semen Fructose		positive	negative
REMARKS: